Protocol for qualitative analysis of lysosome immunoprecipitation from patient-derived glioblastoma stem-like cells - Centre de Recherche en Cancérologie et Immunologie Nantes Angers
Article Dans Une Revue STAR Protocols Année : 2024

Protocol for qualitative analysis of lysosome immunoprecipitation from patient-derived glioblastoma stem-like cells

Résumé

Lysosomes are critical for the sustenance of glioblastoma stem-like cells (GSCs) properties. We present a protocol to enrich and purify lysosomes from patient-derived GSCs in culture. We describe the steps required to stably express a tagged lysosomal protein in GSCs, mechanically lyse cells, magnetically immunopurify lysosomes, and qualitatively assess these organelles. We then detail the procedure for retrieving intact and purified lysosomes from GSCs. We also specify cell culture conditions, storage procedures, and sample preparation for immunoblotting.
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hal-04730342 , version 1 (10-10-2024)

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Clément Maghe, Julie Gavard. Protocol for qualitative analysis of lysosome immunoprecipitation from patient-derived glioblastoma stem-like cells. STAR Protocols, 2024, 5 (2), pp.103121. ⟨10.1016/j.xpro.2024.103121⟩. ⟨hal-04730342⟩
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